Single-particle evanescent light scattering simulations for total internal reflection microscopy
نویسندگان
چکیده
منابع مشابه
Single-particle evanescent light scattering simulations for total internal reflection microscopy.
We simulate and measure light scattering of a micrometer-sized spherical particle suspended in solution close to a glass substrate. The model, based on the discrete sources method, is developed to describe the experimental situation of total internal reflection microscopy experiments; i.e., the particle is illuminated by an evanescent light field originating from the glass-solvent interface. In...
متن کاملTotal internal reflection and evanescent gain.
Total internal reflection occurs for large angles of incidence, when light is incident from a high-refractive-index medium onto a low-index medium. We consider the situation where the low-index medium is active. By invoking causality in its most fundamental form, we argue that evanescent gain may or may not appear, depending on the analytic and global properties of the permittivity function. Fo...
متن کاملSingle-shot super-resolution total internal reflection fluorescence microscopy
20 We demonstrate a simple method for combining instant structured illumination microscopy (SIM) 21 with total internal reflection fluorescence microscopy (TIRF), doubling the spatial resolution of TIRF 22 (down to 115 +/13 nm) and enabling imaging frame rates up to 100 Hz over hundreds of time points. 23 We apply instant TIRF-SIM to multiple live samples, achieving rapid, high contrast super-r...
متن کاملOrientation of the myosin light chain region by single molecule total internal reflection fluorescence polarization microscopy.
To study the orientation and dynamics of myosin, we measured fluorescence polarization of single molecules and ensembles of myosin decorating actin filaments. Engineered chicken gizzard regulatory light chain (RLC), labeled with bisiodoacetamidorhodamine at cysteine residues 100 and 108 or 104 and 115, was exchanged for endogenous RLC in rabbit skeletal muscle HMM or S1. AEDANS-labeled actin, f...
متن کاملTotal internal reflection fluorescence (TIRF) microscopy.
Total internal reflection fluorescence (TIRF) microscopy (TIRFM) is an elegant optical technique that provides for the excitation of fluorophores in an extremely thin axial region ("optical section"). The method is based on the principle that when excitation light is totally internally reflected in a transparent solid (e.g., coverglass) at its interface with liquid, an electromagnetic field, ca...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied Optics
سال: 2006
ISSN: 0003-6935,1539-4522
DOI: 10.1364/ao.45.007299